猪AQP11基因微环表达载体的构建

doi:10.11843/j.issn.0366-6964.2014.01.002

Abstract

Abstract:

This study aimed to construct minicircle expression vector of AQP11 and observe the effect of transfection in Hela cells.AQP11 gene was amplified from the porcine intestinal mucosa and minicircle expression vector minicircle-AQP11 was constructed.The recombinant plasmid minicircle-AQP11 was transfected into Hela cells to investigate the expression of GFP by green fluorescent microscope.The sequencing results showed that the whole sequence of AQP11 gene containing a whole CDS was amplified successfully.Minicircle expression vector minicircle-AQP11 was constructed successfully.GFP was successfully expressed in Hela cells by the detection of bright green fluorescence for one week.Which provide new ideas for further elucidating the biochemical process and functional regulation of AQP11.

CLC Number:

GUO Zhun，GAO Hui-zhen，HAN Li-qiang，GUO Yu-jie，YANG Guo-yu. Construction of a Minicircle Expression Vector of Aquaporin11 Gene in Pig[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 9-13.

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Construction of a Minicircle Expression Vector of Aquaporin11 Gene in Pig

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